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Jonathon Coren, Ph.D. |
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Biographical Information
Education:
B.A. University of Pennsylvania 1983
Ph.D. Cornell University 1991
Professional Experience:
- Elizabethtown College, Associate Professor 2006-present
- Elizabethtown College, Assistant Professor 2002-2006
Courses
- BIO 102 NPS Human Heredity and Inherited Disease
- BIO 211 Genetics
- BIO 412 Seminar in Biology
Research
The Human Genome Project has identified the sequence of several human beings. The technology of constructing human genomic libraries was central to completing this project. Genomic libraries constructed in new human expression vectors are valuable resources for studying any region of DNA in a human background since individual members from these libraries can be rapidly grown in bacteria to obtain workable quantities of DNA and then introduced into human cells to determine their function.
My lab has constructed an 115,000 member human genomic library in the expression vector pJCPAC-Mam2. We have pooled the library members in a rows and column scheme to reduce the library to a more manageable size for screening purposes using PCR to identify desired genes. This resource will be extremely useful for a variety of studies in my lab as well as to the entire human genome community. We plan to study gene expression of the tumor suppressor gene p53, at the levels of transcription and translation to demonstrate the utility of this technology.
Relevant Publications
Gundersen, E. and Coren, J,. 2007. Generating Nested Deletions in PAC Clones
Constructed in pJCPAC-Mam2. BIOS 38: 87-94.
Chatterjee, P. K., Shakes, L. A., Srivastava, D. K., Garland, D. M., Harewood, K. R., Moore, K. J., and Coren, J. S. 2004. Mutually exclusive recombination of wild-type and mutant loxP sites in vitro facilitates transposon-mediated deletions from both ends of genomic DNA in PACs. Nucleic Acids Res. 32: 5668-5676.
Coren, J.S., Sternberg, N., 2001. Construction of a PAC vector system for the propagation of genomic DNA in bacterial and mammalian cells, and subsequent generation of nested deletions in individual library members. Gene 264, 11-18
Chatterjee, P.K., Coren, J.C., 1997. Isolating large nested deletions in PACs and BACs by in vivo selection of P1 headful-packaged products of Cre-catalyzed recombination between the loxP site in PAC and BAC and one introduced in transposition. Nucleic Acids Res. 25, 2205-2212.
Undergraduate Researchers and
Their Current Positions:
Amit Prasad - Penn State College of Medicine
Kate Howell - School Teacher
Erin Gundersen - Penn State College of Medicine
Courtney Knauss- University of Pittsburgh Medical School
Joshua Mundorff- Philadelphia College of Osteopathic Med.
Joe Szulewski - Philadelphia College of Osteopathic Med.
Alicia Alcamo- Ohio State Medical School
Dave Hamel - University of Pittsburgh Medical School
Melissa Marschner - Cleveland Chiropractic School
Yas Nagahama - SUNY Upstate Medical School
Matthew Kochuba - Temple Medical School
Michael Wagner - LECOM Bradenton
Stephanie Bireley - Elizabethtown College Class of 2010
John Fuesler - Elizabethtown College Class of 2011
